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Antibiotic sensitivity

TZ Talyta Galafassi Zarpelon
LG Lúcio Mauro da Silva Guimarães
PA Poliane Alfenas-Zerbini
EL Eli Sidney Lopes
RM Reginaldo Gonçalves Mafia
AA Acelino Couto Alfenas
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Strain sensitivity to 28 antibiotics was assessed using the standard antibiogram method.25 An inoculum sample of 0.1 mL was evenly spread in a Petri dish (9 cm diameter) containing 523 medium, and four Whatman® No.1 filter paper disks (Ø = 0.7 cm) that were previously soaked in the antibiotics to be tested were distributed over the medium. A completely random design containing three replicates per antibiotic was used. After a 48-h incubation, the presence or absence of an inhibition halo was observed.

Copyright and License information:  ©2016 Published by Elsevier Editora Ltda. on behalf of Sociedade Brasileira de Microbiologia.
This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

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