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2.4 UV-visible spectra deconvolution analysis

XG Xiangming Gu
RH Richard Hickey
AR Antara Rath
AP Andre F. Palmer
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Samples containing Hb were analyzed via UV-visible spectroscopy and compared to the UV-visible spectra of pure species of Hb bound to various ligands through spectral deconvolution. The open-source Python package Alchromy (www.alchromy.com) was used to interpret and analyze spectra, and leveraged a nonlinear least squares fitting function of the SciPy package to determine the fraction of various liganded forms of Hb that contribute to the final spectra of the Hb mixture [22].

Copyright and License information:  ©2022 Gu et al
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