Evaluation of the Inflammatory Markers

Patients underwent at least two cycles of immunotherapy, and peripheral blood samples were gathered up to two weeks before treatment. The samples were sonicated in a timely manner and centrifuged for 2 h. Venous blood (2 ml) was collected from the patient under fasting and resting states and injected into a vacuum anticoagulation tube containing dipotassium ethylenediaminetetraacetate (EDTA-K2). Additionally, 3-4 ml venous blood was collected and injected into a separation-promoting gel tube. Patients’ routine blood and blood biochemical parameters were collected. Peripheral blood samples of absolute neutrophil counts (ANCs), lymphocyte counts (ALCs), monocyte counts (AMCs), and platelet counts were recorded to calculate inflammatory markers. The NLR was calculated as ANC/ALC. The PLR was calculated as platelet count/ALC. The MLR was calculated as AMC/ALC. The dNLR was calculated as ANC/(WBC-ANC). The SII was calculated as ANC×platelet count/ALC. Inflammation markers were then categorized by Youden’s index using receiver operating characteristic (ROC) analysis as the cut-off.