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Both Huh7 and Huh7.5 cells were cultured in Dulbecco's modified Eagle's medium (DMEM) supplemented with 10% fetal bovine serum (FBS), 1% penicillin-streptomycin, and 1% nonessential amino acids. HEK293T cells were grown in DMEM containing 5% FBS. Huh7 cells harboring a subgenomic replicon derived from genotype 1b and interferon (IFN)-cured cells were grown as reported previously (12).
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