A total of 14 LADC cell lines (A549, ABC1, HCC827, NCI-H358, NCI-H441, NCI-H522, NCI-H1437, NCI-H1650, NCI-H1975, PC3, PC14, RERF-LC-KJ, RERF-LC-MS, VMRC-LCD) were obtained from the Japanese Collection of Research Bioresources (Ibaraki, Japan), RIKEN BioResource Center (Tsukuba, Japan), or American Type Culture Collection (Manassas, VA, USA) and cultured in appropriate media. To analyze the effects of DNA demethylation and/or histone acetylation on gene expression, cells were treated with 1 μM 5-aza-dC for five days and/or 300 nM trichostatin A (TSA) for the last 12 h of culture.
Do you have any questions about this protocol?
Post your question to gather feedback from the community. We will also invite the authors of this article to respond.
Tips for asking effective questions
+ Description
Write a detailed description. Include all information that will help others answer your question including experimental processes, conditions, and relevant images.