Cell lines

KK Koichiro Kajiura
KM Kiyoshi Masuda
TN Takuya Naruto
TK Tomohiro Kohmoto
MW Miki Watabnabe
MT Mitsuhiro Tsuboi
HT Hiromitsu Takizawa
KK Kazuya Kondo
AT Akira Tangoku
II Issei Imoto
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A total of 14 LADC cell lines (A549, ABC1, HCC827, NCI-H358, NCI-H441, NCI-H522, NCI-H1437, NCI-H1650, NCI-H1975, PC3, PC14, RERF-LC-KJ, RERF-LC-MS, VMRC-LCD) were obtained from the Japanese Collection of Research Bioresources (Ibaraki, Japan), RIKEN BioResource Center (Tsukuba, Japan), or American Type Culture Collection (Manassas, VA, USA) and cultured in appropriate media. To analyze the effects of DNA demethylation and/or histone acetylation on gene expression, cells were treated with 1 μM 5-aza-dC for five days and/or 300 nM trichostatin A (TSA) for the last 12 h of culture.

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