4.5. Cell Viability Assay

The effect of the ADBR2-blockade on the cell viability of primary ccRCC tumors and 786-O cells was performed by analyzing the number of viable cells in the culture, based on the quantification of the presence of ATP, hence indicating metabolically active cells.

First, 5 × 10³ cells/well were seeded in a 96-well plate and incubated in complete medium with [0- 50- and 100-μM] propranolol or ICI for 72 h. Then, 100 μL/well of the Luminescent Cell Viability Assay kit (Promega, Madison, WI, USA) was added and gently mixed for 15 min at RT. Luminescence was measured using a GLOMAX multidetection apparatus (Promega).