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Rat Subdermal BP Implants.

AZ Andrey Zakharchenko
YX Yingfei Xue
SK Samuel Keeney
CR Christopher A. Rock
IA Ivan S. Alferiev
SS Stanley J. Stachelek
HT Hajime Takano
TT Tina Thomas
CN Chandrasekaran Nagaswami
AK Abba M. Krieger
MC Michael Chorny
GF Giovanni Ferrari
RL Robert J. Levy
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Male juvenile (3 wk of age, average weight of 75 g) Sprague-Dawley rats (Charles-River Laboratories) received subdermal BP implants under general anesthesia (isoflurane), as previously published (24). All protocols were approved by the Institutional Committee for the Use and Care of Animals of The Children’s Hospital of Philadelphia. The BP implants were 10 × 10 mm2 glutaraldehyde-fixed BP patches with the following modifications: 1) unmodified; 2) ethanol-pretreated (24 h, room temperature); 3) POZ-modified; 4) ethanol-treated and POZ-modified. Each animal was implanted with one implant from each group, in cohorts of 10 rats per time point. Animals were killed at 7 and 28 d after implantation and harvested explants were rinsed in sterile saline. Half of each explant was either frozen or placed in 10% neutral buffered formalin for histological processing.

Copyright and License information:  ©2022 the Author(s). Published by PNAS.
This open access article is distributed under Creative Commons Attribution-NonCommercial-NoDerivatives License 4.0 (CC BY-NC-ND).

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