MFG-E8 KO C57BL/6 mice were generated, and genotyped as described previously (24, 29, 31). MFG-E8 KO mice were generated by interbreeding homozygous animals carrying the targeted MFG-E8 allele. Interbreeding homozygous C57BL/6 mice and C57BL/6-Tg (CAG-EGFP) mice were purchased from Japan SLC. Eight- to twelve-week-old mice were used for all experiments.
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