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2.7 Quantification of Fluorescence Intensity

JW Jiwon Woo
EL Eunice Yoojin Lee
ML Mirae Lee
SK Seockmo Ku
JP Jeong-Yoon Park
YC Yong Eun Cho
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Quantification of fluorescence intensity was performed using Zeiss ZEN-2 software. Fluorescence intensity as a function of imaging depth was determined by measuring the median signal intensity at z-stack imaging depths (rat: 1500 μm, mouse: 500 µm). The fluorescence signals of single z-stack images were measured as integrated density, using ImageJ software [National Institutes of Health (NIH), MD, United States]. The mean values of the green fluorescence signal of single z-stack images were calculated. The median fluorescence intensity (%) and integrated density (%) were normalized to the intensity of the most superficial slide.

Copyright and License information:  ©2022 Woo, Lee, Lee, Ku, Park and Cho.
This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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