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Protein Analysis

JG Jackie L. Gottshall
VG Vivian A. Guedes
JP Josephine U. Pucci
DB Daniel Brooks
NW Nora Watson
PS Phorum Sheth
AG Ainslee Gabriel
SM Sara Mithani
JL Jacqueline J. Leete
CL Chen Lai
BQ Bao-Xi Qu
CD Christina Devoto
JG Jessica M. Gill
KK Kimbra Kenney
JW J. Kent Werner
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Plasma and EV Levels of IL-6, IL-10 and TNFα were measured using an ultrasensitive assay (Neurology 3-Plex A, item 101995, Quanterix Corporation, Lexington, MA) and single-molecule technology (SIMOA™) on a HD-1 analyzer (Quanterix Corporation) and reported in pg/mL. Samples were randomized over plates and tested in duplicate with laboratory scientists blinded to participant groups. All samples underwent quality control analysis and samples with reported coefficients of variation over 20% were not used for analysis. Samples with high coefficients of variation and concentrations below lower limits of quantification were replaced by values equal to half of the limit of concentration respective to the assay.

Copyright and License information:  ©2022 Gottshall, Guedes, Pucci, Brooks, Watson, Sheth, Gabriel, Mithani, Leete, Lai, Qu, Devoto, Gill, Kenney and Werner.
This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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