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Preparation of tissue homogenate and Western blot analyses for protein levels of NLRP3, ASC, caspase-1, and monocyte chemoattractant protein-1 in the renal medulla.
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Renal medullary tissue homogenates were performed as described previously (46). Primary antibodies used were as above with addition of rabbit polyclonal anti-monocyte chemoattractant protein (MCP)-1 (Abcam). The intensities of the blots were determined using an imaging analysis program (ImageJ, free download from http://rsbweb.nih.gov/ij/).
Copyright and License information: ©2016 the American Physiological Society
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