Glycine-induced LTP

Hippocampal neurons were infected by shRNA virus at DIV 7–10, and subjected for chemical LTP induction at DIV 18–20. Briefly, neurons were firstly incubated in extracellular solution (ECS; in mM: 140 NaCl, 2 CaCl₂, 5 KCl, 5 HEPES, 20 glucose) supplemented with 500 nM TTX, 1 μM strychnine, 20 μM bicuculline (Buffer A; pH 7.4) at 37 °C for 5 min, and treated with 200 μM glycine in Buffer A for 10 min. Then neurons were incubated in Buffer A for another 5–10 min. The cells were then harvested for experiments (Lu et al., 2001; Merriam et al., 2013; Zhang et al., 2015).