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Apoptotic cells in the tumors were detected by terminal deoxynucleotidyl transferase dUTP nucleotide nick end labeling (TUNEL). Briefly, a commercial one-step TUNEL apoptosis assay kit (Beyotime Institute of Biotechnology, Shanghai, China) was used according to the manufacturer's instructions. Cy3-labeled TUNEL positive cells on the sections were detected by confocal microscopy.
Copyright and License information: ©2016 Li et al.
This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
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