Improve Research Reproducibility A Bio-protocol resource
Concise Method
tooltip

Cell lines, cell culture, and materials

CY Chunping Yu
YZ Yi Zhang
NW Ning Wang
WW Wensu Wei
KC Ke Cao
QZ Qun Zhang
PM Peiying Ma
DX Dan Xie
PW Pei Wu
BL Biao Liu
JL Jiahao Liu
WX Wei Xiang
XH Xing Hu
XL Xuewen Liu
JX Jianfei Xie
JT Jin Tang
ZL Zhi Long
LW Long Wang
HZ Hongliang Zeng
JL Jianye Liu
ask Ask a question
Favorite

The bladder cancer (T24) cells were purchased from the ATCC (Manassas, VA, USA) and cultured using Dulbecco’s modified Eagle’s medium (DMEM) supplemented with 10% fetal bovine serum (FBS) at 37 °C in a 5% CO2 incubator. 4′,6-diamidino-2-phenylindole (DAPI) was acquired from Molecular Probes (Eugene, OR, USA). Dojindo Laboratories (Kumamoto, Japan) supplied the cell counting kit 8 (CCK-8), and BD PharMingen (Heidelberg, Germany) supplied the Annexin V-fluorescein isothiocyanate (FITC) Apoptosis Detection kit. Genepharma (Shanghai, China) synthesized and supplied the siRNAs. The circPRMT5-targeting siRNA consisted of a sense strand 5′-AUCUUCCGGCUCCUCAAGUUCTT-3′ and an antisense strand 5′-GAACUUGAGGAGCCGGAAGAUTT-3′. The scrambled negative control siRNA (NC)-siRNA also consisted of a sense strand 5′-UUCUCCGAACGUGUCACGUUU-3′ and an antisense strand 5′-ACGUGACACGUUCGGAGAAUU-3′. The sequence of the FAM and Cy5-labeled (fluorescent) NC-siRNAs was the same, and the dye was attached to the antisense strand.

Copyright and License information: The Author(s) ©2022
Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

Do you have any questions about this protocol?

Post your question to gather feedback from the community. We will also invite the authors of this article to respond.

post Post a Question
0 Q&A