Synthetic Nesocodin NMR.

First, 1 mg of the dried red solid nesocodin was dissolved in one mL of methanol-d₄ containing 0.1% tetramethylsilane as an internal standard and transferred into a 5-mm thin-walled quartz NMR tube (Wilmad 535-PP-7). NMR data including one-dimensional (1D) ¹H-NMR (SI Appendix, Fig. S11), two-dimensional ¹H-double-quantum filtered correlation spectroscopy-NMR (2D ¹H-DQF-COSY) (SI Appendix, Fig. S12), 1D ¹³C-NMR (with and without ¹H decoupling) (SI Appendix, Fig. S13), two-dimensional heteronuclear single quantum coherence-NMR (2D HSQC-NMR) (SI Appendix, Fig. S13B), and two-dimensional Nuclear Overhauser Effect Spectroscopy-NMR (2D NOESY-NMR) (SI Appendix, Fig. S14) were obtained on a Bruker 600 MHz NMR at the University of Minnesota NMR Center on a fee-for-service basis and analyzed using TopSpin version 4.0.6.