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Leaf tissue of 20–25 mg was collected each from control and drought treated plants and were kept in 1 ml of 80% acetone in dark for overnight or till complete extraction of pigment, OD were recorded at 470, 647 and 663 nm for chl a, chl b and carotenoids determination according to Wellburn (1994).
For anthocyanin estimation, leaf samples of 30–35 mg were homoginized in 1% acidified methanol. The absorbance of supernatant was observed at 530 and 650 nm. Using the corrected OD the total anthocyanin content was calculated by (Murray and Hackett 1991).
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