Cell culture and transfection

The American Type Culture Collection (ATCC, USA) provided non-cancerousthyroid cells, HTori-3, and thyroid cancer cells CAL-62. The thyroid cancer cell line CAL-62 was cultured in Dulbecco’s modified eagle’s medium (Sigma-Aldrich,USA) with 1% penicillin/streptomycin and 10% fetal bovine serum (Sigma-Aldrich,USA). They subsequently underwent transfection of oligonucleotides (50 nm, miR-195-5p mimics, NC-mimics,miR-195-5p inhibitor, NC-inhibitor,and si-TERT,respectively), and Lipofectamine 2000 (Invitrogen, Carlsbad, CA) was used according to the manufacturer’s instructions.