Improve Research Reproducibility A Bio-protocol resource
- Home
- Protocols
-
Concise Method
Enzyme-linked immune sorbent assay (ELISA)
Ask a
question
Favorite
ELISA kits (TaKaRa, Dalian, China) were adopted to examine interleukin-1β (IL-1β), interleukin-6 (IL-6) and tumor necrosis factor (TNF-α) levels. Cells were cultured in a 6-well plate at 37°C in 5% CO2 for 24 h and starved for 12 h. Besides, the cell supernatant was subsequently collected, with the concentration of inflammatory cytokines detected according to the manufacturer’s instructions.
Copyright and License information: ©2021 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group.
This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Do you have any questions about this protocol?
Post your question to gather feedback from the community. We will also invite the authors of this article to respond.
Post a Question0 Q&A
