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Lentivirus-shRNA transfection
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The Lenti-shRNA vector system (pGV112) was constructed, packed, purified by GeneChem (Shanghai, China), and manipulated according to the protocol provided by the manufacturer. Then, transfection was performed using Lipofectamine 2000 following the manufacturer’s instructions.
To identify the transfection efficiency, SiHa and HeLa cells were transfected with lentivirus plasmids (sh-DGKZ or sh-NC). 48 h post transfection, DGKZ expression was observed using inverted fluorescence microscope (OLYMPUS, Japan).
Copyright and License information: ©2021 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group.
This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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