Co- infections with N. parisii and P. aeruginosa

Co-infection assays were performed by first pulse infecting co-infection and N. parisii single infection groups with a maximal dose of spores for 3 hr on unseeded 6-cm NGM plates as described above. PA14::DsRed single infections were pulsed with a volume of M9 to match that of the spores. Animals were then washed off in 1 ml of M9 +0.1% Tween-20, followed by two more washes, prior to placement on full lawns of PA14::DsRed on a 6-cm SK plates prepared as described above. N. parisii single infections were placed on a 6-cm NGM plate pre-seeded with 200 μl of 10 x OP50-1. Plates were incubated at 21 °C.