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An Annexin V PE/7-AAD tool kit (Solarbio, Beijing, China) was used to detect apoptosis. The cells were resuspended at a density of 1 × 105/ml. After incubation with Annexin V PE and 7-AAD, according to the manufacturer’s protocols, the cells were detected using a flow cytometer (Verse, BD, USA).
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This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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