2.2. Data preprocessing and analysis of differential expression genes (DEGs) and immune infiltration

Limma was used to analyze the DEGs of sepsis (sepsis_0d) vs control (untreated) and sepsis_ARDS (sepsis-induced ARDS) vs control (untreated) (| logFC | > 1, pval < 0.05). Then, the two differentially expressed genes were intersected. CIBERSORT has advantages in terms of noise, unknown mixture content and closely related cell types, and can characterize cell composition through gene expression profile of complex tissues. As a result, CIBERSORT was used in this study to evaluate the difference of immune infiltration between the sepsis group and the sepsis-induced ARDS group.