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For ASMTL-AS1 knockdown, pre-designed siRNAs targeting ASMTL-AS1 (si-ASMTL-AS1) or LAMC1 (si-LAMC1) and their corresponding negative control (si-NC) were provided by Genepharma (Shanghai, China). The miR-1343-3p mimic, miR-1343-3p inhibitor, mimic-NC and inhibitor-NC were also provided by Genepharma. All cell transfections were performed using Lipofectamine 3000 reagent (Life Technologies, USA). Forty-eight hours post-transfection, HCCLM3 and Huh7 cells were subjected to the following assays.
Copyright and License information: ©2021 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group.
This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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