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cAMP was quantified from liver homogenates using a Direct cAMP ELISA Kit (ab133051; Abcam, Cambridge, UK) according to the manufacturer’s protocol and normalized to total liver weight. Intracellular cAMP concentration of cultured macrophages and HUVECs was quantified 30 min after the start of stimulation using the Direct cAMP ELISA Kit (Enzo Life Sciences, Farmingdale, NY, USA) according to the manufacturer's protocol.
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