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MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) test was performed to study the antiproliferative activity of lettuce by the previously reported method [42] using MCF7 and HePG2 cell lines. For cytotoxicity assay, 7 × 104 cells/well for the MCF7 line and 3.4 × 104 cells/well for HepG2 and HeLa lines were seeded in a 12-well plate, respectively. MTT assay calculated the viability of the cells by taking absorbance at 570 nm after 48 h in a UV2310 spectrophotometer (Dinko, Spain).

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