2.3. Model Preparation and Experimental Method

Ten black/C mice were divided into two groups according to the random number table: control group and experimental group, with five mice in each group. In both groups, CT26 cells in the logarithmic growth phase were inoculated into the spleen to establish a mouse model of CRC. Mice in the control group: 0.3 mL of normal saline was intragastrically administered per day; mice in the experimental group: 0.2 mL of GQD was intragastrically administered per day at a ratio of 0.2 g of medicinal materials/10 g; both groups were intragastrically administered once a day for 10 days and sacrificed and pathological sections were made. Enzyme-linked immunosorbent assay kit was used for both groups of sections (preparation method: known antigens or antibodies were adsorbed on the surface of solid phase carrier (i.e. polystyrene microreaction plate), so that the enzyme-labeled antigen-antibody reaction was performed here. The free components in the liquid phase were removed by washing method). The expression of PI3K/AKT/FOXO1 signaling pathway was detected, and the expression of the ABTB1 gene was also detected by real-time quantitative PCR (extraction and quality detection of total RNA⟶synthesis of cDNA by reverse transcription reaction⟶PCR reaction⟶result analysis) Finally, the rectal cancer cells in the two groups of mice were subjected to scratch assay and transwell assay (steps: preparation of transwell chamber⟶preparation of cell suspension⟶inoculation of cells⟶statistics of results) to observe the expression of p-PI3K, p-AKT, p-FOXO1, and ABTB1 in mice.