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Frozen tissue sections were mounted on slides and allowed to dry. Mounted sections were dehydrated in 100% propylene glycol for 5 min and then stained with ORO for 10 min (Abcam, catalog #ab150678). The sections were then differentiated in 85% propylene glycol for 3 min and rinsed with distilled water 3 times. Coverslips were applied to all slides using an aqueous mounting medium (Vector Laboratories, catalog #H-1400). Stained sections were imaged with a Keyence BZ-X700 digital microscope.
Copyright and License information: ©2022 Becktel et al.
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