Improve Research Reproducibility A Bio-protocol resource
- Home
- Protocols
-
Request a Protocol
Ask a
question
Favorite
We measured apoptotic cells by propidium iodide staining and FACS analysis [15]. Cells were harvested; pelleted by centrifugation; resuspended in phosphate-buffered saline (PBS) containing 50 μg/mL propidium iodide, 0.1% Triton X-100, and 0.1% sodium citrate; and subjected to vortexing prior to FACS analysis (Becton-Dickenson FACScan, Mountain View, CA; FL-3 channel).
Copyright and License information: ©2016 Hao et al.
This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Do you have any questions about this protocol?
Post your question to gather feedback from the community. We will also invite the authors of this article to respond.
Post a Question0 Q&A
