Experiments were repeated three times. Data are presented as the mean ± SD from at least three independent experiments. The results were analyzed using GraphPad Prism 6.0 (GraphPad Software, Inc.) software. Statistical significance of the differences between groups was determined by one-way ANOVA followed by Tukey's post hoc test. The data were estimated from three independent experiments. P<0.05 was considered to indicate statistically significant differences.
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