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Oil-red staining was performed as previous established conditions (Li et al., 2021). Oil-red powder was first dissolved in isopropanol solution (0.5 g/ml). Freshly cut sections from frozen livers and hepatocytes were fixed in 4% formaldehyde, washed in PBS solution and subsequently stained by Oil-red solution for 30 min. The image was captured by Aperio Versa (Leica, Wetzlar, Germany).
Copyright and License information: ©2022 Zhou, Ding, Gu, Fan, Liu, Li, Sun, Wu, Li, Xue, Li and Li.
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