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Adenylation of the 3′-adapter using TS2126 RNL1
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The 3′-DNA adapter (Supplementary Table S5) was adenylated using the TS2126 RNL1 [67] and purified using phenol/chloroform extraction and ethanol precipitation [68]. For in vitro experiments, the non-radioactive adapter was mixed with 5′-labelled adapter in a ratio of 5:1.
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