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3.9. Neuraminidase Activity

CL Carla Monteiro Leal
SL Suzana Guimarães Leitão
LM Leonardo Luiz Oliveira de Mello
IR Isabel de Castro Rangel
CS Carlos Vinicius Azevedo da Silva
MM Milene Dias Miranda
AT Amanda Resende Tucci
CA Camilla Blanco de Assis
CS Carolina de Queiroz Sacramento
NF Natalia Fintelman-Rodrigues
HK Hector Henrique Ferreira Koolen
BV Boniek Gontijo Vaz
RS Rosineide Costa Simas
GL Gilda Guimarães Leitão
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The activity of viral neuraminidase (NA) was measured in the viruses present in the supernatants harvested from the influenza replication inhibition assays using the NA-Star kit (Applied Biosystems, Foster City, CA, USA), according to the manufacturer’s instructions. Briefly, culture supernatants were incubated for 30 min with a chemiluminescent neuraminidase substrate at room temperature, and then an accelerator solution was added triggering light emission from the cleaved substrate. The chemiluminescent signal was read in the luminometer.

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Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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