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One stool sample (1–2 g) was collected from each subject. We provided instructions describing how to collect stool specimens, place them in nucleic acid preservative, and deliver them to our Research Center within 24 to 48 h after collection. Stool specimens were collected in Assay Assure (Sierra Molecular, Incline Village, NV), which does not require refrigeration. DNA was isolated from the stool samples within 48 h of receipt and stored at −20 °C.
Copyright and License information: The Author(s). ©2016
Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
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