At least 3 independent experiments were performed for each assay, unless otherwise stated. For the quantification of Western blot protein bands, Image Studio 3.1 software (LI-COR Biosciences) was used. Data are shown as the mean ± S.D or as the mean ± SEM as stated in the figure legends’. The different methods for statistical comparisons were applied using GraphPad Prism 9 software and are described in the figure legends. The p-values were designated as follows: * p < 0.05; ** p < 0.01, *** p < 0.001. Values of p > 0.05 were considered not significant (n.s.).
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