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We designed three peptide fragments peptide-1: QEEGQVEGQDEDIPITC, peptide-2: CGVEGPKGTGPRGPRG, peptide-3: CGPPGPPGPPGPPGLGGN and immunized mice to obtain P1NP monoclonal antibody. The P1NP-α1 chain was expressed by the Corynebacterium glutamate expression system. Natural P1NP protein was purified from the pleural effusion of untreated tumor patients.
Copyright and License information: ©2022 Shuang Han et al.
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