Improve Research Reproducibility A Bio-protocol resource
- Home
- Protocols
-
Concise Method
C. elegans and RNAi mediated interference
Request a Protocol
Ask a
question
Favorite
Wild type N2 Bristol C. elegans were grown and maintained at 20°C as described.33 The feeding method of RNAi delivery was used to deplete PLK-1, as previously described by Timmons and Fire.34 RNAi plasmids for PLK-1 were obtained from the Geneservice Ltd. C. elegans feeding library.35 E. coli HT115 (DE3) bacteria was transformed with the control or PLK-1 RNAi plasmids. Plating of RNAi and feeding RNAi was described previously.36 The L4440 RNAi vector was used as an RNAi control.
Copyright and License information: ©2016 Taylor & Francis
Do you have any questions about this protocol?
Post your question to gather feedback from the community. We will also invite the authors of this article to respond.
Post a Question0 Q&A
