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Identifying actively translated sORFs
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The actively translated sORFs were determined by RibORF (34), which combines alignment of ribosomal A-sites, three-nucleotide periodicity and uniformity across codons. Only those sORFs longer than 6 aa with a start codon followed by an in-frame stop codon within the lincRNA transcripts, high percentage of maximum entropy (PME > 0.6) and predicted probability > 0.5, were defined as actively translated regions.
Copyright and License information: ©2016 The Author(s) 2016. Published by Oxford University Press on behalf of Nucleic Acids Research.
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