Reagents

Vero E6 cells [CRL-1586, Resource Research Identifier (RRID): CVCL0574] were purchased from American Tissue Type Collection. The following items were purchased from Corning TM: EMEM (10-009-CV), HI fetal bovine serum (FBS) (35-016-VC, and 0.25% Trypsin (25053CI). Pen/Strep (15140-122) was purchased from Gibco. Phosphate-buffered saline (PBS) (SH30256FS) was purchased from HyClone. The following items were purchased from Greiner Bio-One: white 384 well plate (781073), white half area 96-well plate (675083), and white 96-well plate (655083). The custom labeling of MAbs was performed by Columbia Biosciences. Rabbit anti-NP SARS-CoV-2 antibodies R001 (40143-R001, RRID number: AB_2827974), R004 (40143-R004, RRID number: AB_2827975), R019 (40143-R019, RRID number: AB_2827973), and R040 (40143-R040, RRID number: AB_2827976) were purchased from Sino Biological, and mouse anti-NP MAb 1C7C7 was provided by Dr. Tomas Moran and purchased from Leinco (LT7000). The following items were purchased from ThermoFisher: Goat anti-mouse HRP (A16072), goat-anti rabbit HRP (A16104), West Femto substrate (34094), Lipofectamine 2000 (11668019), and Optimem I Reduced Serum Media (31985070). Triton X-100 (100×) was purchased from Sigma Aldrich. The cOmplete ULTRA protease inhibitor (PI) (05892791001) was purchased from Roche.