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Step 2—transplanting into sand (semi-hydroponic sand-based system)

CR Charlotte Rambla
SM Sarah Van Der Meer
KV Kai P. Voss-Fels
MM Manar Makhoul
CO Christian Obermeier
RS Rod Snowdon
EO Eric S. Ober
MW Michelle Watt
SA Samir Alahmad
LH Lee T. Hickey
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The selected plants were carefully extracted from clear pots and transplanted into sand-filled pots (two plants per pot) for root biomass assessment. Pots were placed into containers placed on capillary mats; 15 pots were placed in each container in an RCBD design. Plants were grown in the glasshouse at a constant temperature (17 ± 2 °C) over 24 h with diurnal (12 h) natural light. Plants were watered daily using hydroponic solution (1.50 mL of Cultiplex Extra-Nutrex Grown per litre of water); concentrations were slowly increased according to plant growth: days 1–10: 1.50 mL/L, days 11–17: 2 mL/L, days 18–21: 2.50 mL/L.

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