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Bacterial strains and growth conditions

KK Kateryna Kukil
PL Pia Lindberg
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Escherichia coli DH5 αZ1 (Invitrogen) was used for subcloning and conjugation. Escherichia coli cells were grown in LB medium at 37 °C and supplemented with appropriate antibiotics to the final concentrations in the medium: 50 μg ml−1 kanamycin (Km) or/and 20 μg ml−1 chloramphenicol (Cm) (Sigma, Merk).

Synechocystis sp. PCC 6803, a unicellular glucose-tolerant strain was used in this study. Cultures were grown in BG11 medium [45] with respective antibiotics Km 25 μg ml−1 and/or Cm 20 μg ml−1 at 30 °C under constant light. The optical density of Synechocystis cultures at 750 nm was measured using a Varian Cary 50 BIO spectrophotometer, and an OD750 value of 1 corresponds to approximately 108 cells.

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