Improve Research Reproducibility A Bio-protocol resource
- Home
- Protocols
-
Request a Protocol
Ask a
question
Favorite
Rat aortic ring assay [33] and yolk sac membrane (YSM) assay [34] were performed as described previously to evaluate the angiogenic potential of miR-140-5p using CM from EV, miR-140-5p-KD, or miR-140-5p-OE cells. The experiments were repeated three times. Images of the YSM assay were taken using a stereomicroscope (Nikon, Tokyo, Japan).
The secretion of pro-angiogenic factors was evaluated by ELISA Kit (R&D Systems, Minneapolis, MN, USA) as per the manufacturer’s instructions.
Copyright and License information: ©2021 by the authors.
Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
Do you have any questions about this protocol?
Post your question to gather feedback from the community. We will also invite the authors of this article to respond.
Post a Question0 Q&A
