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CLL cells were isolated from fresh or frozen PBMC using a negative selection kit—EasySep™ Human B Cell Enrichment Kit II Without CD43 Depletion (STEMcell, Saint Égrève, France), according to the manufacturer’s instructions. Purity and viability of the cells after isolation were measured by flow cytometry following staining with CD5/CD19 antibodies and Annexin-V/7-AAD, respectively. For each experiment both values exceeded 95%.
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Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
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