2.11.3. γ. H2AX Analysis

For the analysis of the DNA damage induced by the tested compounds, cells were treated and processed as described for the cell cycle analysis until the fixation step. Fixed cells were then processed as previously described [44,45]. The isotype control (ctrl), purified mouse IgG1 Isotype Control was utilized, the primary antibody was phospho-Histone H2AX (Ser−139) antibody (Millipore, Billerica, MA, USA), and the secondary was the goat anti-mouse IgG (H&L) fluorescein-conjugated, affinity-purified secondary antibody (BD Pharmingen, San Diego, CA, USA). The FCM analysis was performed by Attune NxT Acoustic Focusing Cytometer (Thermo Fisher Scientific, Waltham, MA, USA) and analyzed by using the Attune NxT Analysis Software (Thermo Fisher Scientific, Waltham, MA, USA).