The VB-loaded gel microspheres (containing VB in the core) were obtained by dropping the SA solution (pH 5.0) with vitamin B2 (0.25%, w/v) into the gelation medium. The one-layer VB-loaded gel microspheres shall be denoted as sample L1-VB. Then these microspheres were mixed repeatedly with the same SA solution to form multilayered gel microspheres. The two-layer and three-layer VB-loaded gel microspheres shall be denoted as sample L2-VB and L3-VB, respectively.
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