The BT-483 and T-47D cells were seeded into eight-well glass chamber slides (Thermo Fisher Scientific). The mitochondrial transmembrane potential was measured using the MitoScreen test (Becton Dickinson, Heidelberg, Germany) according to the manufacturer’s protocol. The oxidative stress in the cytoplasm was detected using the CellROXDeepRed test (Thermo Fisher Scientific) according to the manufacturer’s protocol.
Do you have any questions about this protocol?
Post your question to gather feedback from the community. We will also invite the authors of this article to respond.