Models were tested for bias using the Elliptical Joint Confidence Region (EJCR) significance test for the true intercept () and slope () of the univariate linear regression of predicted versus reference values (6):
in this equation and are the estimated intercept and slope of the univariate regression, respectively; and are the coordinate axes variables, is Fisher–Snedecor statistic for 2 and N-2 degrees of freedom at 1-α confidence level (95%); and is the mean squared error, determined as:
where refers to the response values as predicted by the univariate regression.
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