2.3. Investigation of Chromosome Localization, Gene Duplication, and Selection Pressure of LysM-RLK Members

The coding sequence (CDS) of the examined genes was retrieved from the Ensemble Plants database using the biomart program [52] to investigate duplication and selection pressure. Tandem duplication is defined as the duplication of genes on the same chromosome separated by no more than 10 genes [53]. Similarly, two criteria were utilized to detect segmental duplication: the aligned region’s identity had to be larger than 90% and the alignment coverage had to be greater than 90% [54]. Synonymous (Ks) and non-synonymous (Ka) substitution rates were evaluated using DnaSP ver. 5 software [55] to establish the kind of selection pressure. TBtools [56] was used to determine the location of genes on chromosomes and the duplication relationship among them.