2.2.7. Statistical Analysis

Data analysis was done using GraphPad Prism version 9 software (GraphPad Software, La Jolla, CA, USA), and the results are expressed as means ± SD. p-value of <0.05 was considered significant. In vivo studies: The effects of exposure, gene, and their associated interactions on outcome variables, including pulmonary alveolarization, pulmonary angiogenesis, and indices of PH, were analyzed using analysis of variance (ANOVA). If a statistical significance of either variable or interaction was noted by ANOVA, the post hoc Bonferroni test was performed. In vitro studies: The effects of hyperoxia, AMPKα1 knockdown, and their interactions on tubule formation and PCNA expression were analyzed by ANOVA.