2.6. Cell Transfection and Luciferase Activity Detection

Cell transfection was performed using the method described in the Lipo8000 Transfection Reagent, Beyotime Biotechnology, Inc., with some modification. The transfection reagent was configured to 50 µL of Opti-MEM^® (Thermo Scientific, Waltham, MA, USA) Medium, 1 µg of plasmid DNA, and 1.6 µL of Lipo8000™ (Beyotime Biotechnology, Shanghai, China) transfection reagent. After 24 h transfection, the cells were lysed, and firefly luciferase detection reagent and renal luciferase detection working solution were added, respectively, to measure RLU on a multifunctional microplate reader.