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2.4. Electrophoretic Mobility Shift Assays (EMSA)
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EMSA was performed with a synthetic double-stranded oligonucleotide that contains a high affinity MYB binding site [48]. For preparation of nuclear extracts, we used non-transfected HEK293T cells or HEK293T that had been transfected with MYB-CT3 expression vector and were incubated for 16 h in the absence or presence of different concentrations of Bcr-TMP.
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